ImmunophenotypingIImmunophenotypingImmunophenotypingEnglishOncologyChild (0-12 years);Teen (13-18 years)BodySkeletal systemTestsAdult (19+)NA2018-03-06T05:00:00ZOussama Abla, MDDanielle Weidman, MDKarin Landenberg, MD000Flat ContentHealth A-Z<p> Learn how immunophenotyping is performed and how it can determine your child's particular leukemia.</p><p> Immunophenotyping is a technique that helps distinguish between different types of leukemia. For this reason, it can be used to determine whether your child has acute lymphoblastic leukemia (ALL) or acute myeloid leukemia (AML). It also distinguishes between each leukemia subtype. </p><h2> Key points </h2><ul><li>Immunophenotyping can determine whether your child has acute lymphoblastic leukemia (ALL) or acute myeloid leukemia (AML) and distinguish leukemia subtypes. </li><li> This technique can detect the different antigens on bone marrow cells.</li></ul>
ImmunophénotypageIImmunophénotypageImmunophenotypingFrenchOncologyChild (0-12 years);Teen (13-18 years)BodySkeletal systemTestsAdult (19+)NA2018-03-06T05:00:00ZOussama Abla, MDDanielle Weidman, MDKarin Landenberg, MDFlat ContentHealth A-Z<p>Découvrez comment l’immunophénotypage est réalisé et comment cette technique permet d’établir le type particulier de leucémie dont votre enfant souffre.</p><p>L’immunophénotage est une technique qui aide à distinguer les différents types de leucémie. Pour cette raison, on peut s’en servir pour déterminer si votre enfant a la leucémie lymphoblastique aiguë (LLA) ou la leucémie myéloblastique aiguë (LMA). Il distingue aussi le sous-type de leucémie.</p><p>Les cellules de la moelle osseuse expriment certaines protéines sur leur surface, appelées antigènes. Différentes cellules ont différents antigènes. Pour cette raison, les antigènes sur la surface des cellules cancéreuses de la LLA et de la LMA sont différents. L’immunophénotypage détecte ces antigènes et détermine, par exemple, si le cancer touche une cellule lymphoïde ou myéloïde.</p><h2>À retenir</h2><ul><li>L’immunophénotypage permet d’établir si votre enfant souffre d’une leucémie lymphoblastique aiguë (LLA) ou d’une leucémie myéloblastique aiguë (LAM) et de distinguer les sous-types de leucémie.</li><li>La technique permet de détecter les différents antigènes présents sur les sites des cellules de la moelle osseuse.</li></ul>

 

 

Immunophenotyping2838.00000000000ImmunophenotypingImmunophenotypingIEnglishOncologyChild (0-12 years);Teen (13-18 years)BodySkeletal systemTestsAdult (19+)NA2018-03-06T05:00:00ZOussama Abla, MDDanielle Weidman, MDKarin Landenberg, MD000Flat ContentHealth A-Z<p> Learn how immunophenotyping is performed and how it can determine your child's particular leukemia.</p><p> Immunophenotyping is a technique that helps distinguish between different types of leukemia. For this reason, it can be used to determine whether your child has acute lymphoblastic leukemia (ALL) or acute myeloid leukemia (AML). It also distinguishes between each leukemia subtype. </p><h2> Key points </h2><ul><li>Immunophenotyping can determine whether your child has acute lymphoblastic leukemia (ALL) or acute myeloid leukemia (AML) and distinguish leukemia subtypes. </li><li> This technique can detect the different antigens on bone marrow cells.</li></ul><p> Bone marrow cells express certain proteins on their surface, called antigens. Different cells have different antigens. For this reason, the antigens on the surface of leukemic cells in ALL and AML are different. Immunophenotyping detects these antigens and determines, for example, whether the cancer is affecting a lymphoid or myeloid cell. </p> <h2>How is immunophenotyping done?</h2> <p>To find your child's leukemia subtype, leukemic cells from your child's bone marrow sample are labelled with different fluorescent markers. For example, the lab specialist may use a green marker that can only bind to antigens on lymphoid cells and a red marker that can only bind to antigens on myeloid cells. In this case:</p> <ul><li>if your child has ALL, the leukemic cells are lymphoblasts. They will be labelled green.</li> <li> if your child has AML, the leukemic cells are myeloblasts. They will be labelled red.</li></ul> <p>The labelled cells are then run through a special flowing liquid, which has a laser that passes through it. The laser detects the type of fluorescent marker on the leukemic cells.</p> <p>Sometimes, immunophenotyping can be done on a peripheral blood sample — especially when the white blood count is very high. However, bone marrow immunophenotyping is generally more reliable.</p>Immunophenotyping

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